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ORAL PRESENTATION / TAM METİN SÖZLÜ SUNUM

The anti-apoptotic properties of BSO at concentrations of 10, 20, and 40 µg/ml were
assessed by analyzing the expression of the apoptosis-associated gene, caspase-3, using RT-

PCR (Figure 2). Compared with the control group, doxorubicin treatment markedly elevated
the mRNA expression levels of caspase-3 (P<0.001). Conversely, administration of 20 and

40 µg/ml BSO significantly attenuated doxorubicin-induced caspase-3 mRNA expression

(P<0.001 and P<0.01, respectively). The most pronounced reduction in expression was
observed in the cohort that received the highest BSO concentration (40 µg/ml), which was

comparable to that in the control group. In contrast, administration of a low dose of BSO (10
µg/ml) did not significantly alter caspase-3 mRNA expression compared to the doxorubicin

group.

DISCUSSION AND CONCLUSION

In this study, we demonstrated that BSO exerts notable cardioprotective effects in a
DOX-induced cardiotoxicity model using H9c2 cells. Pretreatment with BSO attenuated the

inflammatory response by reducing the expression of TNF-α and NF-κB and inhibited
apoptosis by suppressing caspase-3 mRNA expression. The findings indicate that the

protective effects of BSO is mediated through the modulation of inflammatory and apoptotic
pathways. To our knowledge, this study is the first to demonstrate that blueberry seed oil

confers cardioprotective benefits by inhibiting NF-κB, TNF-α, and caspase-3.

Figure 2. BSO reduces doxorubicin-induced caspase-3 mRNA expression in H9c2 cells.

Statistical comparisons were performed using one-way analysis of variance (ANOVA) followed by Duncan’s
multiple range test. Results are presented as means ± SD. Compared with control group, P<0.05, P<0.01,
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### P<0.001; Compared with DOX group, P<0.05, P<0.01, *** P<0.001. C: Control, DOX: doxorubicin, BSO:
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*
Blueberry seed oil.
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