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ORAL PRESENTATION / TAM METİN SÖZLÜ SUNUM

al., 2022). Starting from day 4, 0.1 ml saline was administered intraperitoneally once weekly
for 5 weeks.

3. Autism + infliximab group: Following the same protocol as the autism group, rats
received 0.1 ml infliximab at a dose of 5 mg/kg intraperitoneally once weekly for 5 weeks,

starting on day 4 (Mohamad et al., 2022; Nageeb et al., 2023).
4. Infliximab group: Rats received 0.5 ml phosphate buffer (0.2 M, pH 7.2) orally for 3

days, followed by weekly intraperitoneal injections of 0.1 ml infliximab (5 mg/kg) for 5 weeks
starting on day 4 (Mohamad et al., 2022; Nageeb et al., 2023).
On day 38, rats were euthanized and their brains were rapidly extracted via

craniotomy using microsurgical scissors and forceps, and placed on ice. Using a scalpel,

olfactory connections, brainstem, and cerebellum were separated. Each brain
hemisphere was divided into right and left hemispheres, and the temporal cortex, located

in the lateral cortical region, was dissected and isolated according to anatomical
boundaries. To prepare 10% tissue homogenates, temporal cortex samples were

homogenized with 0.1 M phosphate buffer (pH 7.4) at 1200 rpm for 2 minutes on ice
using a Bullet Blender Storm (USA). The homogenates were centrifuged at 4000 rpm and

+4 °C for 10 minutes, and supernatants were collected and stored for biochemical
analyses.

Oxidative stress markers including malondialdehyde (MDA), glutathione (GSH),
catalase (CAT), glutathione peroxidase (GPx), and advanced oxidation protein products

(AOPP) were quantified in tissue supernatants using ELISA. The following ELISA kits (ELK

Biotechnology, China) were used: MDA (ELK8612), GSH (ELK8577), CAT (ELK5986), GPx
(ELK2222), and AOPP (ELK0784). Samples and reagents were prepared according to the

manufacturer’s instructions, and optical densities were measured at 450 nm using a
Multiskan SKY Microplate Spectrophotometer (UV/VIS).

Statistical Analysis: All statistical analyses were peformed using SPSS software
(version 20.0, SPSS Inc., Chicago, IL, USA). Data are expressed as mean ± standard

deviation (SD). Differences between groups were analyzed by one-way analysis of

variance (ANOVA) followed by Duncan’s multiple range test for post hoc comparisons. A
P-value of ≤ 0.05 was considered statistically significant.

RESULTS

In the study, statistically significant differences were observed between the groups
for all biochemical parameters evaluated (P<0.05) (Table 1).

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