Page 99 - congress

ORAL PRESENTATION / SÖZLÜ SUNUM

Protective Effects of Cannabis sativa Seed Oil on FGF-1 Expression
and Migration in H₂O₂-Induced L929 Fibroblasts Cell

İrfan ÇINAR Sedat GÖKMEN *
2,
1

1 Kastamonu University, Faculty of Medicine, Department of Pharmacology, Kastamonu, TÜRKIYE
2 Kastamonu University, Faculty of Veterinary, Department of Pharmacology and Toxicology,
Kastamonu, TÜRKIYE

*Correspound Author: sgokmen@kastamonu.edu.tr

Skin lesions pose a significant public health issue as they can greatly affect a patient’s

quality of life. Natural compounds may be cost-effective, widely accessible, and low-side-
effect alternatives for wound healing. This study evaluated the protective capacity of

Cannabis sativa seed oil (CSSO) in modulating Fibroblast Growth Factor 1 (FGF-1) activity and
promoting wound healing in mouse L929 fibroblast cells exposed to H₂O₂. The in vitro

cytotoxicity of CSSO (1–500 µg/mL) and H₂O₂ (0.5 mM) was assessed using the CVDK-8
assay. Migration assays served as an in vitro wound healing model. The experimental groups

were designed as follows: The control group received no treatment. In the H2O2-exposed

group, L-929 cells were exposed to 0.5 mM H₂O₂ for 24 h. In the CSSO treatment groups,
cells were pretreated with increasing concentrations of CSSO (10, 25, 50 and 100 µg/ml) for

3 h, followed by incubation with 0.5 mM H₂O₂ for 24 h. FGF-1 expression was quantified using

Real-Time Polymerase Chain Reaction. The results demonstrated that exposure to H₂O₂
significantly decreased FGF-1 expression compared to that in the control group (P<0.01).

Treatment with 25 µg/mL CSSO significantly increased FGF-1 levels relative to those in the
control group (P<0.05). Notably, CSSO at concentrations of 50 and 100 µg/mL induced a

more pronounced elevation, restoring FGF-1 expression to levels comparable to those in the
control group (P>0.05). Migration assays showed that CSSO at 50 and 100 µg/mL

significantly promoted fibroblast migration into the wound area, with 100 µg/mL achieving
nearly complete wound closure. Our findings revealed that CSSO promoted cell migration

and triggers the expression of FGF-1. Together, these results provide evidence of CSSO's
regenerative potential of CSSO in tissue repair and set the stage for in vivo studies and

clinical trials to confirm in vitro findings.

Keywords: Cannabis sativa seed oil, cell migration, FGF-1, H₂O₂, L-929.

96