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ORAL PRESENTATION / SÖZLÜ SUNUM

Vanillic acid attenuates pyraclostrobin-induced oxidative stress in Drosophila melanogaster

1,*
3
2
Sinan İNCE Fahriye ZEMHERİ NAVRUZ Ali TÜREYEN Gamze ATAMAN 4

1 Afyon Kocatepe University, Faculty of Veterinary Medicine, Department of Pharmacology
and Toxicology, Afyonkarahisar, TÜRKIYE
2 Bartın University, Faculty of Science, Department of Molecular Biology and Genetics,
Bartın, TÜRKIYE
3 Republic of Türkiye Ministry of Health Eskisehir City Hospital, Department of
Gastroenterology, Eskisehir, TÜRKIYE
4 Bartın University, Institute of Science, Department of Molecular Biology and Genetics,
Bartın, TÜRKIYE

*Correspound Author: incesinan@gmail.com

Pyraclostrobin, a strobilurin fungicide, is widely used in the world. In this study, the

protective effect of vanillic acid against pyraclostrobin-induced oxidative stress and its role

in the Nrf2/Keap1 signaling pathway were investigated in Drosophila melanogaster. The
experimental groups were composed of control, pyraclostrobin (250 µg/L), and 0.1, 0.5, and

1 mg/L doses of vanillic acid alone and in combination with pyraclostrobin. Pyraclostrobin
treatment reduced the larval to pupal emergence and the emergence of male and female

adult flies. In addition, increased malondialdehyde levels, whereas glutathione levels and
activities of antioxidant enzymes (SOD and CAT) decreased in pyraclostrobin-treated flies.

Also, pyraclostrobin caused DNA damage in Drosophila melanogaster. In addition,
pyraclostrobin up-regulated the expression levels of oxidative stress-related genes (SOD, CAT,

gclc, Nrf2, Keap1, and hsp70). Vanillic acid treatment prevented larval toxicity and improved
biochemical and oxidative stress-related parameters in flies exposed to pyraclostrobin. In

conclusion, vanillic acid was effective in preventing pyraclostrobin toxicity in Drosophila

melanogaster by enhancing development and reducing oxidative stress via the Nrf2/Keap1
signaling pathway.

Keywords: Drosophila melanogaster, Nrf2/Keap1, oxidative stress, pyraclostrobin, vanillic
acid.

Acknowledgement: This study was financially supported by a grant from the Afyon Kocatepe
University Scientific Research Council of Türkiye (Project No: 23.VF.04).

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