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ORAL PRESENTATION / SÖZLÜ SUNUM

Evaluation of the Effects of Oxytetracycline and Flunixin Meglumine
on the Non-Pregnant Bovine Myometrium Using an Isolated Organ Bath System

Aylin PEHLİVAN ALKAN 1,* Enes ATMACA
1

1 Ondokuz Mayıs University, Faculty of Veterinary Medicine, Department of Veterinary
Pharmacology and Toxicology, Samsun, TÜRKIYE

*Correspound Author: aylinpalkan@gmail.com

The aim of this study was to evaluate the effects of oxytetracycline and flunixin meglumine

on non-pregnant bovine myometrium using an isolated organ bath system. The investigation of
the effects of these pharmacologic agents on uterine contractions aims to provide important

information to improve the efficacy of treatment protocols and contribute to the development of
more effective pharmacotherapeutic strategies for the treatment of postpartum uterine

diseases. In this study, the effects of oxytetracycline (1×10 - 9×10 M) and flunixin meglumine
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(1×10 - 2×10 M) at different concentrations were examined on myometrial contractions using
an isolated organ bath system. Contractions were induced with 0.5 nM oxytocin and myometrial
activity was evaluated by area under the curve (AUC), beats per minute (BPM) and maksimum
peak amplitude (PMAX) parameters. The IC50 values of flunixin meglumine for AUC, BPM and

PMAX parameters were calculated as 3.17×10 , 1.74×10 and 5.41×10 M, respectively, and
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the IC50 values of flunixin meglumine and oxytetracycline for AUC, BPM and PMAX parameters
were calculated as 3.60×10 , 2.03×10 and 7.54×10 M, respectively. According to these
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findings, flunixin meglumine significantly inhibited myometrial contractility over all parameters,
oxytetracycline showed a dosedependent inhibition pattern in BPM and PMAX values while
leading to an increase in AUC value at high concentrations, but this effect was not statistically

significant. When a statistical comparison was made between the groups, it was determined that
flunixin meglumine alone inhibited myometrium activity more strongly at higher concentrations
for AUC (8×10 and 1×10 M) and PMAX (6×10 , 8×10 and 1×10 M) parameters and at lower
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concentrations for BPM (1×10 and 1×10 M), while the inhibition effect decreased when
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applied in combination with oxytetracycline (P<0.05). However, IC50 values obtained from these
three parameters and statistical comparisons between the groups were consistent with each
other in terms of myometrial inhibition effect.
Keywords: Oxytetracycline, Flunixin meglumine, Isolated organ bath, Myometrial contractility,
IC50.

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