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ORAL PRESENTATION / SÖZLÜ SUNUM

Antioxidant and Antiinflammatory Effects of Carvacrol in Rats

with Colistin‐Induced Nephrotoxicity

Yavuz Osman Birdane Orkun Atik Hülya Demirkapı Atik Ruhi Türkmen 1
1
2
1,*

1 Afyon Kocatepe University, Faculty of Veterinary Medicine, Department of Pharmacology
and Toxicology, Afyonkarahisar, TÜRKIYE
2 Afyon Kocatepe University, Faculty of Veterinary Medicine, Department of Physiology,
Afyonkarahisar, TÜRKIYE

*Correspound Author: oatik@aku.edu.tr, orkun1992atik@gmail.com

Colistin (COL) is a polypeptide antibiotic that exhibits bactericidal activity by disrupting
membrane stabilization in bacteria. However, its use is limited due to its nephrotoxic

properties. The spread of infections caused by multidrug-resistant bacteria and the lack and
inadequacy of new antibacterial agents that can be used against these pathogens have led

to the resurgence of COL use. Therefore, preventing the nephrotoxicity (NPT) caused by COL,
which has come back into focus, is a need that has not yet been fully addressed. Carvacrol

(CARV) is a monoterpenoid compound found in the essential oils of aromatic plants such as

thyme, pepper, and wild bergamot. Studies have reported that CARV has antioxidant and
antiinflammatory effects. The aim of this study is to investigate the protective efficacy of

CARV against NPT conditions that limit COL use. A total of 32 rats were used in the study in
four groups of eight rats each, namely, DMSO, COL, CARV, and COL + CARV. COL (300.000

IU/kg/day, intraperitoneal) administered for six days elevated serum BUN, creatinine, uric
acid levels, and renal lipid peroxidation while decreasing renal GSH and the activity of the

antioxidant enzymes SOD, CAT, and GPx. COL also increased the levels of inflammatory
markers NF-κB, TNF-α, and IL-1β, as well as iNOS and COX-2 activities. It increased levels of

KIM-1 and NGAL, markers of acute kidney injury. CARV (75 mg/kg/day, oral) treatment
ameliorated NPT by reducing oxidative stress and inflammation. The findings showed that

CARV attenuated COL-induced NPT.

Keywords: Carvacrol, Colistin, Inflammation, Nephrotoxicity, Oxidative Stress.
Acknowledgement: This work was supported by the Afyon Kocatepe University Scientific

Research Projects Coordination Unit 21.VF.09.

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