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ORAL PRESENTATION / SÖZLÜ SUNUM

Effects of Feed Intake and Water Hardness on
Fluralaner Pharmacokinetics in Layer Chickens

Ataman Bilge SARI¹ * Yiğit GÜNE޹ Ceren ANLA޹ Fulya Üstün ALKAN¹
,
Enes GÜNCÜM² Oya ÜSTÜNER¹ Tülay BAKIREL
1

¹Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Istanbul
University-Cerrahpasa, Istanbul, TÜRKIYE
²Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Kırıkkale
University, Kırıkkale, TÜRKIYE

*Correspound Author: atamanbilge.sari@iuc.edu.tr

Dermanyssus gallinae is an ectoparasite commonly found in layer farms. It causes

anemia, stress, and immunosuppression by sucking blood, leading to significant economic
losses through production/quality loss and the risk of zoonosis. Long-acting agents are

needed for sustainable control; the isoxazoline derivative fluralaner has been reported to
exhibit high long-term efficacy when administered via drinking water to laying hens. In this

study, which investigated for the first time the effects of feed intake status and water

hardness on the pharmacokinetic profile of Fluralaner, thirty-two hens were randomized into
four groups (n=8/group) and given feed+pure water, feed-restricted+pure water, feed-

restricted+hard water (400 mg/L CaCO₃), and feed-restricted+soft water (40 mg/L CaCO₃).
Blood samples were taken from minute 0 to day 21 after administration of Fluralaner (0.5

mg/kg) via drinking water, and plasma concentrations were determined by UHPLC–MS/MS.
Non-compartmental pharmacokinetic parameters were calculated and compared using one-

way ANOVA and Tukey post hoc test. Mean tₘₐₓ was 1.32±0.37 days, Cₘₐₓ was

336.53±90.76 ng/ml, t₁/₂ was 4.00±1.40 days and AUC₀–₂₁ was 1,582.65±374.14 ng/ml
* days. Although Cₘₐₓ was numerically highest in the fed+pure water group, no significant

difference was found between the groups in the parameters Cₘₐₓ, tₘₐₓ, t₁/₂, AUC, MRT₀–

inf_obs, Vz/F_obs and Cl/F_obs (P>0.05).
Our research findings indicate that feed intake status and water hardness did not

significantly alter fluralaner pharmacokinetics or blood distribution; it shows that it supports
flexible application before/after feeding in different water hardness conditions when rearing

poultry.
Keywords: Isoxazoles, pharmacokinetics, fluralaner, poultry, water hardness, feed intake.

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