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ORAL PRESENTATION / SÖZLÜ SUNUM

GLP Requirements and Applications in Cytotoxicity Tests

Yasemin SEZGİN 1,* BEGÜM YURDAKÖK DİKMEN
2

1 Kırıkkale University, Faculty of Veterinary, Department of Pharmacology and Toxicology,

Kırıkkale, TÜRKIYE
2 Ankara University, Faculty of Veterinary, Department of Pharmacology and Toxicology,

Ankara, TÜRKIYE

*Correspound Author: yaseminsezgin@kku.edu.tr

Good Laboratory Practice (GLP) principles provide a structured framework that

ensures reliability, reproducibility, and validity in preclinical safety studies, including
cytotoxicity tests. Compliance with GLP is of particular importance in veterinary drug

research, where candidate compounds must be comprehensively evaluated for safety before

moving on to clinical applications. Cytotoxicity tests conducted on cell lines or primary

cultures are one of the fundamental steps in preclinical toxicological evaluation. These tests

not only provide early information about the potential adverse effects of new molecules, but

also guide dose selection and further in vivo studies. However, if GLP standards are not
strictly adhered to, the results may lose their validity, inter-laboratory comparability, and

acceptability to regulatory authorities. In veterinary medicinal products, which are

compatible with human drug development standards while also taking into account species-

specific considerations, GLP-compliant cytotoxicity tests ensure data integrity, facilitate

international recognition, and reduce the unnecessary repetition of experiments.
Additionally, by improving data quality, GLP contributes to animal welfare in line with the 3R

(Replacement, Reduction, Refinement) principles. This review examines the role of GLP in

cytotoxicity testing and its implications for veterinary drug development.

Keywords: GLP, Cytotoxicity tests, Veterinary drugs.

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