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POSTER PRESENTATION / POSTER SUNUM

Investigation of The Effect of Ursodeoxycholic Acid
Against Liver Damage Induced by Imidacloprid in Rats

Yunus Emre BAŞAR Ebru YILDIRIM Emine BAYDAN
1,*
2
3
Miyase Çınar Tuğçe ANTEPLİOĞLU
5
4

1 Kırıkkale University, Health Scineces Institude, Kırıkkale, TÜRKiYE
2 Kırıkkale University Veterinary Faculty Pharmacology and Toxicology Department ABD,
Kırıkkale, TÜRKiYE
3 Ankara Üniversitesi Veterinary Faculty Pharmacology and Toxicology Department, Ankara, TÜRKiYE
4 Kırıkkale University Veterinary Faculty Biochenistry Department, Kırıkkale, TÜRKiYE
5 Kırıkkale University Veterinary Faculty Pathology Department, Kırıkkale, TÜRKiYE

*Correspound Author: ynsmrbasar@gmail.com

In this study, the toxic effects of imidacloprid (İMD), a widely used neonicotinoid
insecticide, on rat liver were evaluated and the potential protective effect of ursodeoxycholic
acid (UDCA) against this toxicity was investigated in terms of biochemical, and
histopathological parameters. Forty male Wistar albino rats were used in the experiment.
The rats were randomly divided into four groups as the control group (corn oil and 1% gum
arabic administered orally by gavage), the UDCA only group (25 mg/kg/day, administered
orally by gavage), the IMD only group (45 mg/kg/day, administered orally by gavage), and
the combined group (25 mg/kg/day UDCA and 45 mg/kg İMD administered orally by
gavage). All treatments were performed for 30 days, and at the end of the treatments, blood
and liver tissue samples were collected for analysis. In serum biochemical analyses, a
statistically significant increase was observed in serum AST, ALT, GGT, triglyceride, urea, total
cholesterol and total bilirubin levels in the İMD group compared to the control group.
Additionally, a statistically significant decrease was detected in albumin levels of İMD
compared to control group (P<0.001). Statistically significant improvements were recorded
in serum ALT, total bilirubin, triglyceride and urea levels in the group administered with UDCA
(IMD+UDCA) compared to the IMD group. Histopathological evaluations revealed significant
pathological findings of liver injury, such as hepatocyte degeneration, sinusoidal congestion,
necrotic areas, and inflammatory cell infiltration, in the IMD treated group. In the IMD+UDCA
group, the severity of these findings was reduced, and tissue integrity was largely preserved.
In conclusion, it was determined that IMD caused biochemical and histopathological damage
in the liver, while UDCA exhibited a significant protective effect against this toxicity. These
findings suggest that UDCA may be used as a hepatoprotective agent in pesticide-induced
liver injury.
Keywords: Biochemistry, histopathology, imidacloprid, liver toxicity, rat, ursodeoxycholic acid
Acknowledgement: This study was supported by Kırıkkale University Scientific Research
Projects Coordination Unit as master’s thesis project numbered 2024/62.

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