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POSTER PRESENTATION / POSTER SUNUM

Investigation of The Presence of Nitroimidazole Group Drug Residues

Using LC/MS/MS in Raw Milk Consumed Around Ankara

Ebru DİLEK Özgen ÖZDEMİR 2 Sinan İNCE 3,*
1

1 Afyon Kocatepe University, Institute of Health Science, Department of Pharmacology and
Toxicology, Afyonkarahisar, TÜRKIYE

2 Republic of Türkiye Ministry of Agriculture and Forestry, Pendik Veterinary Control
Institute, Toxicology Laboratory, Istanbul, TÜRKIYE

3 Afyon Kocatepe University, Faculty of Veterinary Medicine, Department of Pharmacology
and Toxicology, Afyonkarahisar, TÜRKIYE

*Correspound Author: incesinan@gmail.com

Nitroimidazoles are a group of drugs that are highly active against anaerobic bacteria
and against pathogens of protozoal infections. Their residues in animal products exhibit
mutagenic and carcinogenic effects on humans. In this study, it was aimed to analyze the
presence of residues of nitroimidazole group drugs in raw cow milk samples consumed in
Ankara province with method validation for the determination of nitroimidazole and its
metabolites by liquid-chromatography tandem mass spectrometry (LC/MS/MS) and to
determine the possible residue risk status for both animal health and consumers with the
findings obtained. Pretreatments with ethyl acetate and n-hexane were performed to extract
compounds from the milk samples before analysis. In the final step, the samples were
treated with a methanol-water mixture and injected into the LC/MS/MS system. Analytes
were separated on a C18 column and mobile phase treatment with acetonitrile and formic
acid in water. In the study, it was determined that the recoveries of nitroimidazole
compounds were between 100.5-101.7, detection and measurement limits were between
2.22-2.37 µg/L and 2.66-3.12 µg/L, respectively, and CCα and CCβ values were between
2.21-2.34 µg/L and 2.35-2.58 µg/L, respectively. In addition, 60 raw cow milk samples
collected from Ankara province were analyzed for the presence of nitroimidazole residues by
LC/MS/MS, and it was determined that the samples did not contain nitroimidazole residues
as a result of the data obtained. As a result, it was observed that the method used for the
determination of nitroimidazoles gave sensitive and accurate results, and that the raw milk
offered for consumption did not pose did not pose a health risk in terms of nitroimidazoles.

Keywords: Residue, LC/MS/MS, nitroimidazole, milk.

Acknowledgement: This study was financially supported by a grant from the Afyon Kocatepe
University Scientific Research Council (Project No: 23.SAĞ.BİL.21).

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